Can I work with cell culture media or complex buffer compositions?

Q: Can I work with cell culture media or complex buffer compositions?

RT-IC measurements can be run in any media or buffer of your choice, just make sure to avoid pH indicator dyes such as phenol red. In order to ensure good cell viability throughout the assay it is important to keep them at a physiological pH at all times. Since the heli X cyto does not include an incubator, we recommend using a well buffered solution (e.g. PBS based) for your cell suspension that is not dependent on CO 2 for maintaining a physiological pH.

Question

Bogdan Dochia · Accepted Answer

RT-IC measurements can be run in any media or buffer of your choice, just make sure to avoid pH indicator dyes such as phenol red. In order to ensure good cell viability throughout the assay it is important to keep them at a physiological pH at all times. Since the heliX^cyto does not include an incubator, we recommend using a well buffered solution (e.g. PBS based) for your cell suspension that is not dependent on CO₂ for maintaining a physiological pH.