heliXcyto
Instrument and Chip Handling
The cell traps are made from a bio-compatible polymer. If polymer-interaction is of concern, they can be passivated with a layer of BSA protein.
heliXcyto chips can be re-used many times. An automatic regeneration step is washing out your trapped cells after each measurement. Additional chip cleaning by an optional Chip wash is recommended. Extensive cleaning is also possible manually on the bench with our Chip cleaning kit. You only need to switch to a new heliXcyto chip if your background fluorescence exceeds the cut-off of 3 x 105 cps in the red channel or 4 x 105 cps in the green channel in the Cyto chip test and cannot be lowered by a manual chip cleaning anymore, or if the traps got lost.
Different maintenance scripts for the heliXcyto are provided in heliOS. Optimally, you include a Cyto chip wash after each measurement and 1-3 System washes at the end of the entire experiment. Regular Clean & Sleep routines of the heliXcyto keep the device clean.
To assure chip longevity, handle it with care and avoid extreme temperature jumps. Do not freeze the heliXcyto chip. After use or if you observe increasing fluorescence background counts (above 3 x 105 cps), clean the chip with the Chip cleaning kit and store it at 4 °C in the fridge. You can find further handling tips on the information card delivered together with the heliXcyto chips.