heliXcyto
Real-Time Interaction Cytometry (RT-IC)
Real-Time Interaction Cytometry (RT-IC) is a novel technology applying principles of biophysics and cytometry to enable the measurement of real-time kinetics directly on cells. The unique heliXcyto chip allows for the instantaneous and reversible immobilization of any eukaryotic cell type on the chip surface based on mechanical retention. After the trapping of single cells, association and dissociation of fluorescently labelled analytes can be monitored in real-time due to highly sensitive fluorescence detection with single-photon counters.
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The following parameters can be measured from analytes binding directly to target cells:
Association rate kon
Dissociation rate koff
Affinity, avidity, stability, Kd, t1/2
Fluorescence signal amplitude Fcps, relative target density
You can analyze any analyte of your choice with RT-IC, from small molecules to cytokines to bispecific antibodies to antigen-MHC complexes. The only prerequisite is that it needs to be fluorescently labelled.
No, RT-IC measurements can only be performed with a heliXcyto biosensor. The heliX+ is optimized for the measurement of stably immobilized molecules on the biochip. The heliXcyto has to fulfil the requirements to measure living cells and therefore, specific cytometry-related adjustments needed to be integrated into the biosensor concerning fluidics and configuration. These changes make sure that you will get the accuracy and precision you need to measure kinetics on cells while at the same time providing the necessary conditions to protect precious cell samples. Contact us for attractive package deals for both instruments.
Yes, you can differentiate affinity and avidity and even quantify the contribution of different binding modes, for example binary and ternary binding modes of antibodies.