Bleaching of the fluorophore is reduced by: a very low excitation LED power the usage of stable fluorophores Dyes used in our labeling kits show hardly any signal drift of bulk fluorophore in the microfluidic channel over 1 hour continuous excitation. However, you...
RT-IC can discern off-rates < 1 s-1.
Currently, this is not possible.
RT-IC measurements can be run in any media or buffer of your choice, just make sure to avoid pH indicator dyes such as phenol red. In order to ensure good cell viability throughout the assay it is important to keep them at a physiological pH at all times. Since the...
No, the heliXcyto does not include an incubator. We recommend using a well buffered solution for your cell suspension, that is not dependent on CO2 buffering. Furthermore, you can adjust the temperature of the sample tray to any temperature between 4 °C – 40...
