Yes, you can differentiate affinity and avidity and even quantify the contribution of different binding modes, for example binary and ternary binding modes of antibodies.
No, RT-IC measurements can only be performed with a heliXcyto biosensor. The heliX+ is optimized for the measurement of stably immobilized molecules on the biochip. The heliXcyto has to fulfil the requirements to measure living cells and therefore, specific...
You can analyze any analyte of your choice with RT-IC, from small molecules to cytokines to bispecific antibodies to antigen-MHC complexes. The only prerequisite is that it needs to be fluorescently labelled.
The following parameters can be measured from analytes binding directly to target cells: Association rate kon Dissociation rate koff Affinity, avidity, stability, Kd, t1/2 Fluorescence signal amplitude Fcps, relative target density
Real-Time Interaction Cytometry (RT-IC) is a novel technology applying principles of biophysics and cytometry to enable the measurement of real-time kinetics directly on cells. The unique heliXcyto chip allows for the instantaneous and reversible immobilization of any...
