The cell traps are made from a bio-compatible polymer. If polymer-interaction is of concern, they can be passivated with a layer of BSA protein.
It is recommended to reproduce measurements generated on single-trap chips multiple times to get a good estimate of cell-to-cell-variability. Data obtained on multiple single cells can be plotted into one graph to get an overview of your cell population....
The automated workflow of the heliXcyto includes snapshots of the electrodes before and after cell immobilization, after the dissociation as well as after trap regeneration. These can be evaluated during data analysis.
The heliXcyto has a green and a red detection channel. You can use any low-bleaching fluorochrome with an excitation wavelength in the range of 490-510 nm or 605-625 nm and an emission wavelength in the range of 525-575 and 655-685 nm, respectively.
Labeling of your analyte can be done either by direct conjugation of a fluorescent dye or by secondary detection. We offer a labeling kit based on amine-reactive NHS-ester dyes. Alternatively, you can use any other labeling method of your choice, keeping the detection...
