Bleaching of the fluorophore is reduced by:
- a very low excitation LED power
- the usage of stable fluorophores
Dyes used in our labeling kits show hardly any signal drift of bulk fluorophore in the microfluidic channel over 1 hour continuous excitation. However, you might consider performing specific controls when measuring slow dissociations over many hours. Our application specialists are happy to discuss specific solutions with you. Contact us here.
Category:
Experimental Considerations