Each heliXcyto module allows an automated throughput of about 15 full kinetic experiments, depending on chosen contact times. You can scale this up according to your specific high-throughput needs by combining several modules in one network. Contact us for heliXcyto...
Bleaching of the fluorophore is reduced by: a very low excitation LED power the usage of stable fluorophores Dyes used in our labeling kits show hardly any signal drift of bulk fluorophore in the microfluidic channel over 1 hour continuous excitation. However, you...
RT-IC can discern off-rates < 1 s-1.
Currently, this is not possible.
RT-IC measurements can be run in any media or buffer of your choice, just make sure to avoid pH indicator dyes such as phenol red. In order to ensure good cell viability throughout the assay it is important to keep them at a physiological pH at all times. Since the...